Figure 3.

Specific ⁸⁶Rb⁺ efflux (ordinate; percentage of control) was determined for human α4β4-nAChRs expressed by SH-EP1-hα4β4 cells in the presence of ligand alone (graphs on the left; normalized to responses to 1 mM carbamylcholine) or in the presence of an ~EC₉₀ concentration of carbamylcholine (200 µM; graphs on the right) as described in Materials and Methods. Symbol key: 11 (◇); 13 (▼); 10 (×); AMOP-H-OH (●); 12 (■); 18 (◆); 20 (△); 16 (▽); 26 (+). Log molar EC₅₀ or log molar IC₅₀ values (± S.E.) and agonist efficacies or residual activity in the presence of inactivating drug (± S.E.) are provided in Table 1. Molar EC₅₀ or IC₅₀ values and Hill coefficients are provided inSupplementary Data Table 1.