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. 2010 Oct 15;5(10):e13417. doi: 10.1371/journal.pone.0013417

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Giampà et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Representative photomicrographs of DAB immunohistochemistry for the marker of the medium spiny neurons, calbindin-28K (CALB, black), in the striatum of a (A) wild-type mouse, (B) vehicle treated R6/2 mouse, and (C) R6/2 mouse treated with TP10 from 4 to 13 weeks of age. There is a marked decrease in neuronal count in the sample from a vehicle treated R6/2 mice compared to wild-type mice and TP10 treated R6/2 mice. (D) Quantification of the number of striatal neurons labeled with CALB. Striatal neuron counts were carried out (see Methods) on sections from wild type and vehicle- or TP-10-treated R6/2 mice (n = 6/group). An ANOVA indicated an overall significant effect of group (F(2,90) = 36,48; p<0.0000). Post hoc analysis indicated that R6/2 mice treated with vehicle had a significantly reduced density of CALB-positive neurons in striatum compared to the wild type group. The density of striatal neurons in R6/2 mice treated with TP-10 was not statistically different to that of wild type mice, and was significantly greater than that of the vehicle treated R6/2 animals (p<0.0001). (E) Area of striatal neuron cell bodies labeled with CALB. Cell body area of approximately 400 neurons per animal (n = 6/group) was measured using Zeiss LSM software. An ANOVA indicated an overall significant effect of group (F(2,899) = 61,64; p<0.0000). Post hoc analysis indicated that R6/2 mice treated with vehicle had a significantly reduction in the size of striatal neurons compared to wild type animals (p<0.00002). The size of striatal neurons in R6/2 animals treated with TP-10 was not statistically different to that in wild type animals, and was significantly greater than that of the vehicle treated R6/2 animals (p<0.00002).