Table 1. Primers, annealing temperatures and number of cycles used for the amplification of each target gene.

Target gene	Oligonucleotide primers	Annealing temperature (°C)	Number of cycles	Product size (bp)
beta-actin	F: 5′CCAAAGCCAACCGTGAGA 3′R: 5′ CCAGAGGCGTACAGGGACA3′	50	40	103
granzyme b	F: 5′ AAGTTGGTGCGGTGGGTT3′R: 5′ AGGGTTATAGTCTGGGTGATGG3′	50	40	169
nk-lysin	F: 5′ GCCTCATCTGTGAGTCTTGTCG3′R: 5′ CAGTGTCCTCGTTGGGTTGTG3′	50	40	78
tnfα	F: 5′TGGCCCCTTGAGCATCA AC3′R: 5′CGACGGGCTTATCTGAGGTTT3′	50	40	71
ifnγ	F: 5′ TGGTAGCTCTGGGAAACTGAATG3′R: 5′ TGGCTTTGCGCTGGATCTG3′	50	40	80
Il12p40	F: 5′ GATGCTGGCCAGTACACC3′R: 5′ TCCAGCACGACCTCAATG3′	50	40	111
Il4	F: 5′ GGACACAAGTGCGACATCA3′R: 5′ GCACGTGTGGTGTCTGTA3′	50	40	186
Il10	F: 5′ CGGCGCTGTCATCAATTTCTG3′R: 5′ CCCCTCTCTTGGAGCTTGCTA3′	50	40	89
cxcl12	F: 5′ TACAGATGCCCTTGCCGATT3′R: 5′ TGTTGCTCTTCAGCCGTGC3′	58	40	118
igfbp3	F: 5′ CGAGGAGGACCGCAGTGTA3′R: 5′ TGGCGTGCCCTTTCTTGAT3′	58	40	122