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. 2010 Oct 15;5(10):e13410. doi: 10.1371/journal.pone.0013410

Figure 1. Pluripotency and radiosensitivity of human induced pluripotent stem (iPS) cells.

Figure 1

(A) Western blot analysis of NANOG and OCT4 demonstrating no change in protein level after irradiation. β-actin served as the loading control. (B) Confocal microscopy for NANOG in iPS cells at indicated time periods after irradiation. Inset – zoomed region of a colony displaying nuclear localization of NANOG. Green – NANOG, Blue – DNA, scale bar = 100 µm (20 µm inset). (C) Confocal microscopy for SSEA-4 in iPS cells at indicated time periods after irradiation. Green – SSEA-4, Blue – DNA, scale bar = 20 µm. (D) Western blot analysis for cleaved caspase-3 after irradiation. Human iPS cells were irradiated, or left untreated, returned to the incubator and recovered for the indicated periods of time. Adherent and detached cells were collected and analyzed separately. β-actin served as the loading control. Note that the level of cleaved caspase-3 increases after irradiation, particularly in detached cells.