Table 1. Flow cytometry analysis of the expression of cell adhesion molecules in endometrial epithelial cells and cell lines.
Antigen | EEC | HEC-1-A | RL95-2 |
Negative control | 3.2±0.9 | 3.6±1.9 | 2.2±0.8 |
Integrin β1 | 184±33 | 170±44 | 79±23* vs EEC and HEC-1-A |
Integrin α1 | 7.8±3.2 | 6.3±2.4 | 3.7±0.37 |
Integrin α2 | 49±9 | 32±8 | 21±6 |
Integrin α3 | 46±15 | 52±13 | 22±6 |
Integrin α4 | 3.8±0.5 | 4.6±0.8 | 5±1.2 |
Integrin α5 | 6.3±1.2 | 11±4 | 2.3±0.3 |
Integrin α6 | 42±7 | 133±36* vs EEC | 100±29 |
Integrin αVβ3 | 17±2.4 | 21±1.2 | 11±3 |
Integrin β2 | 3.2±0.4 | 3.8±0.2 | 2.2±0.5 |
ICAM-1 | 36±12 | 33±5 | 11±0.5 |
ICAM-2 | 3.3±0.2 | 4.3±0.5 | 1.8±0.3 |
ICAM-3 | 3±0.2 | 5.3±1.3 | 2±0.5 |
VCAM-1 | 3.9±0.4 | 5±0.75 | 2.7±0.7 |
CD31 | 3.8±0.3 | 6±2.4 | 2.5±0.2 |
CD43 | 3±0.4 | 4.8±1.5 | 1.8±0.7 |
CD44 | 38±15 | 19±8 | 18±3 |
CD59 | 148±10 | 57±10 | 41±5 |
MHC-I | 394±76 | 45±9* vs EEC | 59±4* vs EEC |
E-Selectin | 2.2±0.2 | 5.2±1.4 | 1.8±0.3 |
P-Selectin | 2.7±0.3 | 4.3±1.1 | 1.3±0.1 |
L-Selectin | 2.9±0.1 | 4.6±1.2 | 1.8±0.3 |
CD15 | 91±47 | 4.5±0.7 | 15±2.3 |
PSGL-1 | 3±0.2 | 5.3±1.2 | 2±0.2 |
CD147 | 68±27 | 36±8 | 100±12* vs EEC and HEC-1-A |
CD98 | 50±10 | 54±11 | 307±37** vs EEC and HEC-1-A |
CD9 | 201±50 | 157±44 | 148±32 |
CD81 | 19±2.6 | 109±13 | 20.5±0.5 |
CD151 | 32±3.8 | 33±8.6 | 10.6±0.9 |
Data shown are means ± s.d. of the mean fluorescence intensity (MFI) of n = 2 for integrins α4 and β1, ICAM-2 and 3; VCAM-1, CD43, Selectins, PSGL-1; n = 5 for integrins β1, α2 and α3, ICAM-1, CD147 and CD98. n = 4 for the remaining molecules. For optimal statistical analysis cytometer detectors were set to include autofluorescence basal signal into the first log of the scale. * p<0.005 **p<0.001, ANOVA Newman-Keuls Multiple Comparison Test.