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. 2010 Oct 15;5(10):e13380. doi: 10.1371/journal.pone.0013380

Table 1. Flow cytometry analysis of the expression of cell adhesion molecules in endometrial epithelial cells and cell lines.

Antigen EEC HEC-1-A RL95-2
Negative control 3.2±0.9 3.6±1.9 2.2±0.8
Integrin β1 184±33 170±44 79±23* vs EEC and HEC-1-A
Integrin α1 7.8±3.2 6.3±2.4 3.7±0.37
Integrin α2 49±9 32±8 21±6
Integrin α3 46±15 52±13 22±6
Integrin α4 3.8±0.5 4.6±0.8 5±1.2
Integrin α5 6.3±1.2 11±4 2.3±0.3
Integrin α6 42±7 133±36* vs EEC 100±29
Integrin αVβ3 17±2.4 21±1.2 11±3
Integrin β2 3.2±0.4 3.8±0.2 2.2±0.5
ICAM-1 36±12 33±5 11±0.5
ICAM-2 3.3±0.2 4.3±0.5 1.8±0.3
ICAM-3 3±0.2 5.3±1.3 2±0.5
VCAM-1 3.9±0.4 5±0.75 2.7±0.7
CD31 3.8±0.3 6±2.4 2.5±0.2
CD43 3±0.4 4.8±1.5 1.8±0.7
CD44 38±15 19±8 18±3
CD59 148±10 57±10 41±5
MHC-I 394±76 45±9* vs EEC 59±4* vs EEC
E-Selectin 2.2±0.2 5.2±1.4 1.8±0.3
P-Selectin 2.7±0.3 4.3±1.1 1.3±0.1
L-Selectin 2.9±0.1 4.6±1.2 1.8±0.3
CD15 91±47 4.5±0.7 15±2.3
PSGL-1 3±0.2 5.3±1.2 2±0.2
CD147 68±27 36±8 100±12* vs EEC and HEC-1-A
CD98 50±10 54±11 307±37** vs EEC and HEC-1-A
CD9 201±50 157±44 148±32
CD81 19±2.6 109±13 20.5±0.5
CD151 32±3.8 33±8.6 10.6±0.9

Data shown are means ± s.d. of the mean fluorescence intensity (MFI) of n = 2 for integrins α4 and β1, ICAM-2 and 3; VCAM-1, CD43, Selectins, PSGL-1; n = 5 for integrins β1, α2 and α3, ICAM-1, CD147 and CD98. n = 4 for the remaining molecules. For optimal statistical analysis cytometer detectors were set to include autofluorescence basal signal into the first log of the scale. * p<0.005 **p<0.001, ANOVA Newman-Keuls Multiple Comparison Test.