Table 1.

FLT3 mutations among 517 acute leukemia subjects from the Northern California Childhood Leukemia Study

Patient ID	ITD or MUT*	MUT	Age	Cytogenetics	FAB (lineage)	Backtrack result†
0004	ITD		6.1	46, XY [10/20]; 46, XY, del(9)(p13) [9/20]; 47, XY, +?22 [1/20]	ALL	neg
0087	ITD		10.5	46, XY [21/24]	AML-M1	neg
0104	ITD		9.1	46, XY [19/20]; 44, XY, -14, -22 [1/20]	ALL-L1 (T-cell)	neg
0126	ITD		14.9	46, XX [20/20]	AML-M2
0201	MUT	GAT→GAA D835E	13.2	46, XY [20/20]	AML-M2
0261	ITD		13.7	46, XX, t(6;9)(p23;q34) [23/24]	AML-M2	neg
0544 ‡	MUT	GAT→GTT D835V	5.3	46, XX [20/20]; nuc ish 12p13(TEL×2), 21q22(AML1×4) [149/207]/12p13(TEL×2), 21q22(AML×2) [31/207]/12p13(TEL×2), 21q22(AML1×3) [25/207]	ALL	neg
0678	ITD		14.5	46, XX [20]	AML-M2
0738	MUT	TAT→TGT Y842C	5.0	45, XY, -7, del(13)(q13q21) [12/20]; 46, XY [8/20]	ALL-L1
0745	MUT	GAT→TAT D835Y	12.7	46, XY [21/21]; nuc ish 4cen(CEP4×2), 10cen(CEP10×2), 12p13(TEL×2), 21q22(AML1×2)	ALL-L1/L2
0796 ‡	MUT	GAT→TAT D835Y	1.8	46, XY [3/3]; nuc ish 12p13 (TEL×3), 21q22(AML1×4) [90/100], 12p13(TEL×2), 21q22(AML1×2) [10/100] FISH: +12++21/++X (presumed cryptic high hyperdiploidy)	ALL-L1	neg
0803	MUT	GAT→TAT D835Y and GAT→CAT D835H	0.3	46, XY [20/20]; nuc ish 11q23 (MLL5'x2, MLL3'x2) [200/200]	AML-M5
0945 ‡	MUT	GAT→TAT D835Y	7.9	46, XY [21/21]	ALL	neg
0999	ITD		8.3	46, XX [20]	AML
1043	MUT	GAT→CAT D835H	14.0	46, XY, inv(16)(p12q22) [12/12]	AML
1073 ‡	DEL		5.9	46, XY [30]; nuc ish 9q34(ABL×2), 22q11.2(BCR×2)	ALL	neg
1107 ‡§	MUT	GAT→GCT D835A	3.5	56~58, XY, dup(1)(q21q32),+4,+5,+6,+10,+14,+18,+18,+19,+21, +22,+2mar [5/23]; 46, XY [18/23]	ALL-L1
1148	ITD		14.5	47, XX, +14 [16]	AML	neg

ITD, internal tandem duplication; MUT, point mutation; DEL, deletion.

^† neg: 240 ng of patient Guthrie card was tested and was determined to be negative. The rest of the patients were not tested.

^‡ Patients exhibiting high hyperdiploidy by FISH assay (see Materials and Methods)

^§Patient 1107 has a KRAS mutation, which was also negative in backtracking experiment (ref #23)