Table 2.

PCR Primers used for FLT3 ITD Backtracking on Guthrie card DNA: Northern California Childhood Leukemia Study

Primer Name	Primer Sequences‡ (5'(3')
ITD-R2	AGACAAATGGTGAGTACGTGCA
ITD-4-F	ATATGATCTCtccgagggcc
ITD-87-F	TATGAATATGAgTTCAGAGAATATG
ITD-104-F	GAGTTTCCcctcgggaag
ITD-261-F	AGAGAATATGAGACCGGCTC
ITD-1073-F*	GAGTACTTCccCAGAGAATATG
ITD-1148-F	AGAGAATATGAgTACGTTGATTTC

^‡ bold, WT sequence; underlined, ITD; lower case, N nucleotides

*1073 had an 11 bp deletion with a 2 bp insertion, giving an overall deletion of 9 bp.

ITD-R2 was used as the reverse primer for all the backtracking reactions.