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. 2010 Sep 23;9:257. doi: 10.1186/1476-4598-9-257

Figure 2.

Figure 2

Immunoblotting was used for verification of the results obtained by Pepchip analysis. Band heights were all corresponding to the manufacturer's datasheets (P-Src: 2 bands between 56-61 kDa, Src: 60 kDa, Casein Kinase 2: 42 kDa, P-p65: 65 kDa and p65: 65 kDa). Both cell lines and primary cultures showed phosphorylation of Src and slight variation in amounts of total Src. Casein Kinase 2 and p65 protein were present in all samples in comparable amounts, as is phosphorylated p65, indicating active NF-kappaB signaling.