Table 1.
Sample ID | Type | Gender | Age | P/R/M | Location | FUS/DDIT3 transcript size | FUS/DDIT3 transcript type | (COBRA) FISH | |
---|---|---|---|---|---|---|---|---|---|
1 | L1187 | primary culture | F | 20 | P | left hamstrings | 1033 bp | X | t(12;16) |
2 | L1357 | primary culture | M | 50 | P | left hamstrings | 654 bp | I | t(12;16) |
3 | L1434 | primary culture | F | 43 | P | right hamstrings | 654 bp | I | t(12;16) |
4 | L2187 | primary culture | F | 42 | P | left thigh, subcutaneously | 378 bp | II | N/A |
5 | 402-91 | cell line | M | unknown | unknown | unknown | unknown | N/A | t(12;16) |
6 | 1765-92 | cell line | unknown | unknown | unknown | unknown | unknown | N/A | t(12;16) |
Primary cultures of samples L1187, L1357, L1434 and L2187 were obtained from fresh tumors. Tumors L1187 and L1357 were high grade (> 5% round cell component), whereas L1434 and L2187 were low-grade (< 5% round cell component). These differences in grade however were not reflected in growth rate of the primary cultures (doubling times of primary cultures were all ~ 4 days; doubling times of cell lines ~ 2 days).