Table 3.
Top lists of activated kinases and pathways in different conditions
| top five activated kinases | top activated signalling pathway | |
|---|---|---|
| normal medium condition (including all kinases in the analysis) | ck2a1 | NF-kappaB |
| lck | Src | |
| fyn | RAR | |
| fgr | PPAR | |
| yes | ||
| normal medium condition (leaving out cell cycle related kinases in the analysis) | ck2a1 | NF-kappaB |
| lck | Src | |
| fyn | RAR | |
| camk2b | PPAR | |
| prkcd | ||
| starved medium condition | mapk14 | NF-kappaB |
| ck2a1 | RAR | |
| akt1 | p53 | |
| egfr | G1/S transition of the cell cycle | |
| erbb2 | oxidative stress response | |
Whereas activated kinases differed between cells grown in normal medium (RMPI supplemented with 10% inactivated calf serum), top five of activated signaling pathways were identical. Results were identical for cell lines and primary cultures. Cell lines cultured in starved medium conditions revealed a different top list of both kinases and activated signaling pathways. However, in all three different conditions, NF-kappaB was the most activated signaling pathway identified.
prkcd: protein kinase C, delta; mapk14: mitogen-activated protein kinase 14; egfr: epidermal growth factor receptor; erbb2: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2