Figure 3.

Methylation analysis in L1210 murine model. Panel A. Analysis of murine XPG promoter and identification of putative CpG islands from which primers for methylation specific PCR were designed. Panel B. Methylation specific PCR in L1210 sensitive (wt) and resistant cells (MMDX). M: methylated, U: unmethylated. Panel C. Expression of XPG mRNA and protein in L1210 parental and L1210/MMDX cells untreated or treated for 72 hours with 50 nM AZA. Panel D. Growth inhibitory activity of nemorubicin in L1210 parental (black circle) and L1210/MMDX (black square) cells without (up) or with (bottom) 50 nM AZA. Cells were pretreated with AZA for 72 hours before the addition of nemorubicin.