Table 2.
Proteins exhibiting increased phosphorylation in N2a58/22L cells.
| No. | Uniprot | Protein Names | Ratioa | Pept.b | Sequence Coverage [%] |
PEPc | Biological Process |
|---|---|---|---|---|---|---|---|
| 76 | P80316 | T-complex protein 1 subunit epsilon | 1.017 | 1 | 6.1 | 8.63E-18 | protein folding |
| 77 | Q9CX22 | Putative uncharacterized protein; Cofilin-1 | 1.0173 | 5 | 29.7 | 3.72E-41 | cytoskeleton; protein phosphorylation |
| 78 | P32067 | Lupus La protein homolog | 1.02 | 2 | 7.5 | 1.68E-02 | RNA processing |
| 79 | A6ZI44 | Fructose-bisphosphate aldolase | 1.0328 | 8 | 12.2 | 2.75E-25 | glycolysis |
| 80 | P35700 | Peroxiredoxin-1 | 1.0342 | 6 | 6.7 | 2.44E-10 | proliferation; redox homeostasis; stress response |
| 81 | P05202 | Aspartate aminotransferase, mitochondrial | 1.0344 | 1 | 12.3 | 1.04E-11 | aspartate biosynthesis; oxaloacetate metabolism |
| 82 | Q3TFD0 | Serine hydroxymethyltransferase | 1.0425 | 3 | 6.5 | 2.95E-03 | carbon metabolism |
| 83 | P63101 | 14-3-3 protein zeta/delta | 1.0478 | 19 | 19.2 | 7.09E-27 | protein binding |
| 84 | Q9CZ30-1 | Obg-like ATPase 1 | 1.0606 | 3 | 7.1 | 2.25E-16 | ATP/GTP binding; hydrolase activity |
| 85 | P06745 | Glucose-6-phosphate isomerase | 1.0652 | 3 | 7.7 | 3.34E-15 | angiogenesis; gluconeogenesis; glycolysis |
| 86 | Q71H74 | Collapsin response mediator protein 4A | 1.0688 | 5 | 6.3 | 6.94E-12 | nervous system development |
| 87 | Q6P5F9 | Exportin-1 | 1.0703 | 2 | 3.6 | 2.74E-20 | nuclear export; centrosome duplication |
| 88 | Q01853 | Transitional endoplasmic reticulum ATPase | 1.0708 | 2 | 3.7 | 2.97E-05 | apoptosis; retrograde protein transport |
| 89 | Q3TCI7 | L-lactate dehydrogenase | 1.0746 | 4 | 18.3 | 1.43E-32 | glycolysis |
| 90 | P08113 | Endoplasmin | 1.08 | 1 | 2.6 | 1.05E-05 | protein folding |
| 91 | Q8VC46 | Ubc protein;Ubiquitin | 1.0848 | 17 | 30.7 | 2.18E-06 | protein binding |
| 92 | A0PJ96 | Mtap1b protein | 1.0871 | 2 | 3.2 | 2.72E-13 | cytoskeleton |
| 93 | Q61171 | Peroxiredoxin-2 | 1.0934 | 2 | 9.1 | 4.16E-04 | signal transduction; redox homeostasis |
| 94 | Q9WVA4 | Transgelin-2 | 1.106 | 2 | 11.8 | 9.37E-05 | muscle organ development |
| 95 | P52480-1 | Pyruvate kinase isozymes M1/M2 | 1.1081 | 3 | 22.2 | 6.30E-39 | glycolysis |
| 96 | O08709 | Peroxiredoxin-6 | 1.1204 | 5 | 13.8 | 1.10E-04 | redox homeostasis |
| 97 | P17182 | Alpha-enolase | 1.128 | 11 | 41.5 | 6.35E-26 | glycolysis |
| 98 | P54227 | Stathmin | 1.1422 | 4 | 18.1 | 1.94E-02 | cell cycle; cytoskeleton |
| 99 | Q922F4 | Tubulin beta-6 chain | 1.1427 | 1 | 19.2 | 1.01E-25 | cytoskeleton |
| 100 | Q60864 | Stress-induced-phosphoprotein 1 | 1.1499 | 1 | 3.5 | 5.12E-05 | stress response |
| 101 | P52480-2 | Pyruvate kinase isozymes M1/M2 | 1.2958 | 1 | 19.6 | 3.40E-33 | glycolysis |
| 102 | Q920E5 | Farnesyl pyrophosphate synthetase | 1.3534 | 1 | 8.2 | 8.44E-16 | cholesterol/isoprenoid biosynthesis |
| 103 | P38647 | Stress-70 protein, mitochondrial | 1.357 | 2 | 4.6 | 6.57E-08 | nuclear export; protein folding |
| 104 | P14824 | Annexin A6 | 1.6252 | 4 | 7.9 | 2.47E-08 | Ca2+ transport; muscle contraction |
| 105 | Q61753 | D-3-phosphoglycerate dehydrogenase | 1.7927 | 3 | 8.1 | 6.28E-15 | cell cycle; neural development; serine biosynthesis |
a. Ratio of N2a58/22L vs. N2a58# cells
b. Number of identified peptides
c. posterior error probability (PEP) estimates the probability of wrong assignment of a spectrum to a peptide sequence