Table 3. The effect of the cand-1 mutation on cullin RNAi phenotypes.

Strain	no RNAi	cul-1 RNAi	cul-2 RNAi	cul-3 RNAi	cul-4 RNAi
wild type (RNAi on L4)	0% Emb, 0% Lva (n=100)	84% Emb (n=278)	88% Emb (n=298)	74% Emb (n=258)	5% Emb, 95% Lva (n=262)
cand-1(tm1683) (RNAi on L4)	22% Emb 12% Lva (n=180)	100% Emb (n=160)	96% Emb (n=156)	97% Emb (n=105)	68% Emb, 32% Lva (n=115)
wild type (RNAi on L1) (n=20)	100% became adults with 0% Emb, Lva progeny	100% became adults with 100% Emb progeny	100% became adults with 100% Emb progeny	100% became adults with 100% Emb progeny	100% became adults with 100% Emb progeny
cand-1(tm1683) (RNAi on L1) (n=20)	100% became adults with 22% Emb 12% Lva (n=180)	80% larval arrest, 20% sterile adults	100% became adults with 100% Emb progeny	100% became sterile adults	100% became adults with 100% Emb progeny

L4- or L1-stage larvae were placed on cullin RNAi plates, n=20 each. L4 larvae placed on RNAi all developed to the adult stage, and the F1 progeny were scored for phenotype. L1 larvae placed on RNAi were scored for their own phenotypes. Phenotypes: Lva, larval arrest; and Emb, embryonic lethal. Note that a sterile adult is a more severe defect than an adult that is not sterile but produces Emb progeny.