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. 2010 Sep 25;17(1):78. doi: 10.1186/1423-0127-17-78

Figure 4.

Figure 4

Representative graph showing Real Time amplification of 5-HT2A mRNA from the hippocampus of control and experimental rats. are mean ± S.E.M of 4-6 rats in each group. Each group consists of 6-8 rats. a p < 0.001 when compared to control group, b p < 0.001 when compared to diabetic group. The relative ratios of mRNA levels were calculated using the ΔΔCT method normalized with β-actin CT-value as the internal control and Control CT-value as the calibrator.