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. 2010 Sep 23;5:15. doi: 10.1186/1750-2187-5-15

Figure 3.

Figure 3

Functional AMPK activity is required for activation of Akt at Ser473 and Thr308 in ALL cell lines. CCRF-CEM and NALM6 cells were exposed to either the AMPK activator AICAR (100 and 200 μM) or the AMPK inhibitor compound-C (CompC, 2.5 and 5.0 μM) for 24 h at 37°C. Proteins were extracted and analyzed by Western immunoblotting for the expression of P-Akt (Ser473 and Thr308), P-AMPK (Thr172), and P-ACC (Ser79). The density value of each band was normalized to β-actin level and expressed relative to control (shown as fold induction).