Figure 2.

Genetic organizations of the reconstituted OtAGE of strain Ikeda and the largest OtAGE found in strain Boryong. Both strains contain a large number of OtAGEs, but extensive decay has occurred in all OtAGE copies. A possible original structure of Ikeda OtAGE was reconstituted by comparing larger fragments in our previous report.¹³ The Ikeda OtAGE shown in this figure was constructed by combining OtAGE_178 and OtAGE_40. Genes for conjugal transfer are depicted by green boxes, those for integrase by red, those for transposase by yellow, those for ATPase domain-containing proteins by blue, conserved hypothetical genes by white and OT-specific hypothetical genes by gray. All genes in the OtAGEs are drawn to the scale. Numbers above the reconstituted Ikeda OtAGE indicate family numbers of OtRG families. Numbers below the Boryong OtAGE indicate CDS numbers in Boryong. Homologous regions indicated by light green shading were identified by the BLASTN program (E-value threshold: ≤1E−10). Note that the Boryong OtAGE contains several deletions and IS insertions, suggesting that this largest OtAGE copy is also defective.