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. 2010 Sep 22;81(9):093704. doi: 10.1063/1.3483263

Figure 2.

Figure 2

Dye partitioning and phase transition in a GUV. For this sample, SSM/DOPC/cholesterol=0.23∕0.27∕0.50. The black and white images depict the two different fluorescence channels at 24.600 °C prior to merging and false color assignment. The upper black-and-white image shows the LR-DPPE fluorescence (Ld phase) and the lower image shows naphthopyrene fluorescence (Lo phase). The middle image shows an equatorial section of a GUV with Lo-Ld phase coexistence at 24.600 °C and the right image shows the GUV at 35.300 °C, after it has undergone a miscibility transition. The red false color denotes the Ld phase probe LR-DPPE and the blue false color denotes the Lo phase probe naphthopyrene.