Table 2.

List of well types randomly loaded on each 96 well plate. One of the seven DNA hairpins (j=1–7) under study was loaded into each experimental signal well. The number of replicates of each well type per plate is the number of well numbers, k, corresponding to that experimental condition.

Well type	Well contents			Well number, k
	Buffer A	SYBR Green I dye	DNA hairpin
Control buffer well	1X	0	0	kbuffer=1–4
Control dye well	1X	2X	0	kdye=5–12
Experimental signal well j	1X	2X	2.2 μM stem base pairs	kDNA=1+12j