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. 2010 Aug 20;61(15):4325–4338. doi: 10.1093/jxb/erq234

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© 2010 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 2. — Xylosyltransferase activity of recombinant GAGT. Left panel: GAGT cDNA was expressed in Pichia pastoris cells under the control of a methanol-inducible promoter (see the Materials and methods). Extracts from the uninduced (lane 1) and methanol-induced cells (lane 2) were incubated with UDP[¹⁴C]-xylose and GA. Lane 3 corresponds to UDP[¹⁴C]-xylose. Right panel: samples of UDP-xylose (lane 1), standard GA-5-O-β-D-xyloside, previously obtained in our laboratory (lane 2; Fayos et al., 2006) and the GA xyloside produced using the crude tomato leaf extracts (lane 3) were separated by TLC under the same conditions, and were chemically revealed as described in the text.