Table 1.
Purification of GAGT from tomato leaves
| Step | Total protein | Total activity | Specific activity | Purification | Yield |
| (mg) | (milliunits) | (milliunits mg−1) | (Fold) | (%) | |
| Crude extract | 527.4 | 7700.1 | 14.60 | 1.0 | – |
| (NH4)2SO4 (35–65%) precipitation | 301.3 | 6544.2 | 21.72 | 1.5 | 84.9 |
| Q-Sepharose, first step | 3.7 | 1852.1 | 500.56 | 34.3 | 24.1 |
| Q-Sepharose, second step | 1.1 | 1082.4 | 983.96 | 67.4 | 14.1 |
| HiTrap Blue | 0.2 | 199.5 | 997.46 | 68.3 | 2.6 |
GAGT was extracted from 100 g of tomato leaves and activity was measured by integrating the HPLC fluorescence peak area corresponding to the GA-xyloside as detailed in the Materials and methods.