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. Author manuscript; available in PMC: 2010 Oct 15.
Published in final edited form as: Dev Dyn. 2008 Jul;237(7):1767–1779. doi: 10.1002/dvdy.21587

Figure 4.

Figure 4

In situ hybridizations for Six2 in whole-mount embryos. In E8.5 (A-C), Six2 was detected primarily in the first brachial arch of wild type embryos (A), with a noticely reduced expression in Br/+ embryos (B), and a further reduction in Br/Br embryos (C). At E11.5 (D-F), Six2 is expressed in the midface, facial prominences, first branchial arch, and the urogenital region of the wild type (D). The Br/+ embryo shows significantly reduced expression (E), and Six2 expression in the Br/Br embryo is nearly absent (F). However, note the activation of Six2 expression in the lens of the Br/+ (E) and more so in Br/Br (F) embryos. At E12.5 (G-L), Six2 expression remains obvious in the cranial base region and facial prominences of wild type heads (G), while reduced in the Br/+ heads (H) and absent in the Br/Br heads (I), with the exception of the lens. Also at E12.5, Six2 expression was strong in wild type kidneys (J), much reduced in Br/+ kidneys (K), and absent in Br/Br kidneys (L).