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. Author manuscript; available in PMC: 2010 Oct 15.
Published in final edited form as: Dev Dyn. 2008 Jul;237(7):1767–1779. doi: 10.1002/dvdy.21587

Figure 6.

Figure 6

Real time qPCR and Western blot analysis showing that Six2 is significantly down-regulated in Br/+ and Br/Br tissues. qPCR measured Six2 mRNA levels in RNA extracted from the head and torso of E10.5–11.0 +/+, Br/+, and Br/Br mice (A). Expression of Six2 in Br/+ and Br/Br embryos is shown relative to expression of Six2 in +/+ embryos after being normalized against the amount of EF1α1. Error bars correspond to one standard deviation. Western blotting with an anti-Six2 antibody for proteins extracted from the head and kidney of E13.5 +/+, Br/+, and Br/Br mice (B) also shows a descending magnitude of Six2 protein in mutant mice. Quantities of total proteins in each lane were confirmed by probing with an anti-β-actin antibody. The net intensities of each Six2 Western blot band was measured and normalized with the β-actin net intensity for each sample, confirming quantitative decreases in Six2 protein levels in Br/+ and Br/Br mutant embryos (C). Real time qPCR measured no changes in Six3 mRNA expression levels in E11.5 Br/+ and Br/Br mutant embryo heads (D). * = p-value < 0.01.