CB1 and FAAH mRNA expression in TIP39-KO and WT mice. Dark field images of emulsion dipped brain sections following in situ hybridization with 35S-riboprobes directed against CB1 or FAAH mRNA. Images from WT animals are in the left column and TIP39-KO mice in the right column. CB1 expression can be seen in the hippocampus (A, B) and in the amygdala (C, D). FAAH expression is shown in the hippocampus (E, F) and amygdala (G, H). Quantifications of the expression levels are shown at the right (I–N). O) Change in CB1 expression is plotted against change in FAAH expression, with each symbol indicating measurement of the two mRNA's on adjacent sections from one animal (Linear regression, Goodness of fit, F = 27, r2 = 0.65, P<0.001). **P<0.01, ***P<0.0001. Abbreviations: BLA – basolateral amygdaloid nucleus, anterior; BMA – basomedial amygdaloid nucleus, anterior; CA1 – field CA1 hippocampus; CA3 – field CA3 hippocampus; CeL – central amygdaloid nucleus, lateral; CeM – central amygdaloid nucleus, medial; D3V – dorsal 3 rd ventricle; DG – dentate gyrus; opt – optic tract. Scale bar – 100 μm.