Figure 5. The average number of spikes evoked during von Frey filament stimulation of the forepaw increases with respect to the filament strength and depends on the order of application.
A significant interaction effect was found between the von Frey magnitude and the ordinal rank of the stimulus application (p<0.0001); at greater filament magnitudes, firing was greater in response to the first application of the filament relative to the subsequent applications at that magnitude.