. Author manuscript; available in PMC: 2010 Oct 16.

Published in final edited form as: Food Chem Toxicol. 2007 Jun 15;45(12):2359–2365. doi: 10.1016/j.fct.2007.06.012

Table 2.

Inhibition of human P450_2E1 by isolated isobutylamides from E. purpurea and S. acmella (the total reaction volume was 0.500 mL).

Isobutylamide from figure 1	[p-nitrophenol]	Enzyme	% inhibition
I @ 0.030 mM	0.020 mM	HLM	50 %
I @ 0.030 mM	0.020 mM	Expressed 2E1	53 %
II @ 0.025 mM	0.020 mM	HLM	40 %
II @ 0.025 mM	0.020 mM	Expressed 2E1	62 %
IX + X @ 0.125 mM	0.020 mM	HLM	40 %
IX + X @ 0.125 mM	0.020 mM	Expressed 2E1	53 %
Spilanthol @ 0.067 mM	0.020 mM	HLM	55%
Spilanthol @ 0.067 mM	0.020 mM	Expressed 2E1	75 %