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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Nature. 2010 Sep 2;467(7311):108–111. doi: 10.1038/nature09318

Figure 1. Reconstitution and 5′ polarity of DNA end resection.

Figure 1

a, Resection of uniformly labeled DNA. Symbol: HD, heat denaturation of DNA.

b, Time course analysis of resection with 3′-labeled (i) or 5′-labeled (ii) DNA. Mean values ±SD from three independent experiments were plotted (iii). The asterisk in (i) and (ii) marks the area that was quantified.

c, Analysis of the initial 5′ resection product in a denaturing polyacrylamide gel.

d, The biotinylated substrates (i) were incubated with the resection machinery for 5 min as in (a) and analyzed (ii). Complete digestion of the unblocked 5′ strand yielded ssDNA (designated P in (ii) and depicted in (iii)) that harbored biotin-streptavidin at the 5′ terminus and 32P at the 3′ terminus.