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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Nature. 2010 Sep 2;467(7311):108–111. doi: 10.1038/nature09318

Figure 2. Requirement for Sgs1 helicase and Dna2 nuclease activities.

Figure 2

a, The dna2-D657A (DA) mutant and mutant MRX harboring mre11-3 (11-3) were tested with 3′-labeled substrate. Symbol: HD, heat-denaturation of DNA.

b, The dna2-K1080E (KE) and sgs1-K706A (KA) mutants were tested.

c, Srs2 helicase, Fen1 nuclease, or their combination failed to substitute for Sgs1 and Dna2. Symbol: DA, dna2-D657A.

d, RAD27 over-expression (YEp24) failed to suppress the end resection defect of dna2Δ cells. Initial resection next to the break (MAT probe) and long-range resection (FEN2 probe) were analyzed6, as depicted (i). A set of primary data and the mean values ±SD from three independent experiments are presented (ii).