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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Nature. 2010 Sep 2;467(7311):108–111. doi: 10.1038/nature09318

Figure 4. Role of Top3 and RPA in resection.

Figure 4

a, Top3-Rmi1 and top3-Y356F-Rmi1 were tested in the resection system with 3′-labeled substrate. Symbol: Bl, no protein added.

b, Analysis of 5′ resection in top3Δ cells supplemented with the low copy pRS315 vector carrying TOP3 or top3-Y356F gene. Analysis was done as in Figure 2d.

c, Crossover (CO’s) and noncrossover (NCO’s) recombinants are distinguished based on their size (i). The frequencies of CO’s 8 hr after break induction were determined7 and the mean values ±SD from three independent experiments were plotted (ii).

d, SSB or hRPA supports DNA unwinding but not end resection. Symbol: HD, heat denaturation of DNA.

e, Yeast RPA stimulates 5′ end digestion (i) but attenuates 3′ end digestion (iii) by Dna2. The mean values ±SD from three independent experiments were plotted in (ii) and (iv).