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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: J Immunol. 2009 Dec 16;184(2):1022–1030. doi: 10.4049/jimmunol.0901945

Figure 2. Prx1 stimulates dendritic cell maturation and activation.

Figure 2

(A and B) Immature bone marrow derived dendritic cells (iBMDCs) were incubated with media alone, 20–200 nM Prx1 or 100 nM LPS for 24h. (A) Following incubation cells were analyzed by flow cytometry for expression of CD11c and CD86. Results are shown as percent total cells; error bars represent standard deviation. (B) Supernatants were harvested and analyzed for TNF-α. Results are shown as pg/ml and are representative of three independent experiments; error bars represent standard deviation. (C) TG-elicited macrophages were incubated with media harvested from prostate tumor cell lines that were transfected with cDNA encoding for either control shRNA (Scramble) or shRNA specific for Prx1 (shPrx1) or in media harvested from cells expressing Prx1 specific shRNA to which 50 nM exogenous Prx1 had been added (shPrx1 + Prx1). Following 24h incubation, supernatants were harvested and analyzed for TNF-α. Results are shown as pg/ml and are representative of three independent experiments; error bars represent standard deviation. **: P≤0.01 when compared to TNF-α levels secreted by cells incubated with media alone; ##: P≤0.01 when compared to TNF-α levels secreted by cells incubated with media from cells expressing control shRNA; ††: P≤0.01 when compared to TNF-α levels secreted by cells incubated with media from cells expressing shRNA specific for Prx1.