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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: J Immunol. 2009 Dec 16;184(2):1022–1030. doi: 10.4049/jimmunol.0901945

Figure 3. Prx1 induced cytokine secretion is TLR4 dependent.

Figure 3

(A) iBMDCs were isolated from C57BL/6 (TLR4+/+; open bars) and C57BL/10ScNJ (TLR4−/−; closed bars) mice and stimulated with 200 nM Prx1, 100 nM LPS, or 100 mM Pam3Cys. Supernatants were collected and analyzed by IL-6 ELISA kits. (B) TG-elicited macrophages were isolated from C57BL/6 (TLR4+/+; open bars) and C57BL/10ScNJ (TLR4−/−; closed bars) mice and stimulated with 200 nM Prx1, 100 nM LPS, or 100 mM Pam3Cys. Supernatants were collected and analyzed by IL-6 ELISA kits. Results are presented as pg/ml; error bars represent standard deviation; asterisks indicate P values less that 0.01. (C) Naïve C57BL/6 (TLR4+/+; open bars) and C57BL/10ScNJ (TLR4−/−; closed bars) mice were injected i.p. with 200 nm Prx1. Six hours later, blood was collected and analyzed by ELISA for the presence of IL-6. Results are presented as pg/ml; error bars represent standard deviation; asterisks indicate P≤0.0002.