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. Author manuscript; available in PMC: 2010 Oct 16.
Published in final edited form as: Immunity. 2006 Nov 30;25(6):929–940. doi: 10.1016/j.immuni.2006.10.012

Figure 1.

Figure 1

The Generation of Mice Homozygous for a Disrupted Ndfip1 Gene

(A) The location of the gene trap vector within the Ndfip1 locus is shown. The Ndfip1 locus contains 8 exons spanning about 45 Kb of genomic DNA.

(B) The gene trap vector was inserted into the second intron and results in the production of a truncated Ndfip1 mRNA that encodes exons 1 and 2.

(C) Offspring resulting from the intercross between Ndfip1+/− mice were tested by PCR to identify Ndfip1−/− animals via the primer pairs shown in Figure 1A.

(D) To confirm the disruption of Ndfip1, RNA was isolated from Ndfip1+/+ and Ndfip1−/− mice and RT-PCR was performed. We detect the presence of two transcripts in Ndfip1−/− tissues, Ndfip1-AST and Ex2-βGEO. GADPH is shown as a reverse transcriptase and loading control.