Skip to main content
. Author manuscript; available in PMC: 2010 Oct 16.
Published in final edited form as: Neuron. 2008 Oct 23;60(2):353–366. doi: 10.1016/j.neuron.2008.08.027

Figure 7. Time Dependency of Memory Degradation during One-Day Contextual Recall and Cued Recall.

Figure 7

Tg = 12. WT = 12. The amount of freezing over each 30 s was pooled into one time point. The total measurement time was 3 min.

(A and B) For contextual recall at 0.5 min (p = 0.396); at 1 min (p = 0.039); at 1.5 min (p = 0.037); at 2.0 min (p = 0.0067); at 2.5 min (p = 0.0029); and at 3 min (p = 0.039) (A).

For cued recall at 0.5 min (p = 0.728); at 1 min (p = 0.008); at 1.5 min (p = 0.03); at 2.0 min (p = 0.0026); at 2.5 min (p = 0.004); and at 3 min (p = 0.025) (B). Degradation/erasure of the first training day memory evidenced from lack of savings on the second training trials. The first recall took place without NM-PP1 injection. The second and third trainings and recalls occurred under NM-PP1 treatment so that Tg-CaMKII-F89G was suppressed. Freezing at each test reflects the cumulative memory formation from the previous training day(s).

(C and D) Cumulative formation of contextual fear memories from three consecutive days of trainings. For the contextual memory recall, in the ANOVOA by effect of group: first test day F(1,22) = 5.01, p = 0.035; second day F(1,22) = 5.76, p = 0.025, < 0.05. There is also a significant genetic groups × day interaction: F(5,66) = 4.27, p = 0.002, < 0.01. There is no significant difference between the amount of freezing in the day one test of wild-type mice and day two test of transgenic mice, indicating no savings from the first conditioning memory in Tg mice.

(E and F) Cumulative formation of cued fear memories from three consecutive trainings. For the cued freezing, in the ANOVOA by effect of group: first test day F(1,22) = 5.67, p = 0.026; second day F(1,22) = 4.51, p = 0.045, < 0.05. There is also a significant groups × day interaction: F(5,66) = 6.50, p = 0.0006. However, there is no significant difference between the amount of cued freezing in day one test of wild-type mice and day two test of transgenic mice, suggesting erasure of one-day memory in Tg mice.

All values are mean ± SEM.