Table 2.

Steady-state kinetic parameters of wild-type and mutant PAMO

Mutant	k cat (s−1)		K M (μM)		k cat/K M (mM−1 s−1)		(k cat/K M)NADPH/(k cat/K M)NADH
	NADPH	NADH	NADPH	NADH	NADPH	NADH
WT	3.1	0.39	0.7	560	4,000	0.70	6,000
R217A	2.2	0.25	320	1,900	7.0	0.13	54
R217L	0.8	0.22	850	1,440	1	0.15	7
T218A	3.8	0.76	1.4	1,110	2,700	0.68	4,000
H220A	3.3	0.51	0.7	510	5,000	1.0	5,000
H220D	1.2	0.76	36	780	33	0.97	34
H220E	1.2	0.28	170	710	7.0	0.39	20
H220F	1.9	0.25	20	750	95	0.33	290
H220N	3.6	0.59	0.6	260	6,000	2.3	3,000
H220Q	2.7	0.35	1.7	150	1,600	2.3	700
H220T	2.9	0.43	1.7	800	1,700	0.54	3,100
H220W	0.8	0.19	2.3	860	300	0.22	1,000
K336H	1.2	0.22	11	1,100	110	0.20	550
K336N	1.6	0.39	1.1	910	1,500	0.43	3,500
H220Q/K336H	1.9	0.67	11	2,400	170	0.28	610
H220Q/K336N	1.0	0.36	2.4	810	420	0.44	950