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PAMO-catalyzed sulfoxidation of thioanisole using NADPH or NADH
| ||||||
|---|---|---|---|---|---|---|
| Mutant | NADPH | NADH | ||||
| t (h) | c (%)a | ee (%)b | t (h) | c (%)a | ee (%)b | |
| WT | 24 | 84 | 44 | 30 | 14 | 42 |
| R217A | 24 | 30 | 41 | 30 | 7 | 44 |
| T218A | 24 | 51 | 42 | 30 | 6 | 48 |
| H220E | 24 | 39 | 39 | 48 | ≤3 | – |
| H220N | 24 | 77 | 45 | 30 | 37 | 46 |
| K336N | 24 | 45 | 58 | 30 | 7 | 64 |
a Determined by GC
bDetermined by HPLC
