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PAMO-catalyzed kinetic resolution of racemic 3-methyl-4-phenylbutan-2-one using NADPH or NADH
| ||||||
|---|---|---|---|---|---|---|
| Mutant | NADPH | NADH | ||||
| t (h) | c (%)a | E c | t (h) | c (%)a | E c | |
| WT | 1.5 | 51 | 47 | 3 | 32 | 55 |
| R217A | 1.5 | 34b | 55 | 6 | ≤3 | – |
| T218A | 1.5 | 53 | 49 | 3 | 17 | 59 |
| H220E | 3 | 23b | 51 | 6 | ≤3 | – |
| H220N | 1.5 | 43 | 51 | 3 | 17 | 63 |
| K336N | 1.5 | 33 | 92 | 6 | 17 | 96 |
aDetermined by GC
b[NADPH] = 500 μM
cEnantiomeric ratio, 
