Ex vivo gamma interferon (IFN-γ) ELISPOT assays were performed as described in Materials and Methods. Individual cytomegalovirus (CMV) phosphoprotein 65 (pp65) T-cell responses are shown for CMV-seronegative subjects vaccinated with 1 mg (A), or 5 mg (B) doses of VCL-CB01 at 0, 2, and 8 weeks and CMV-seronegative subjects vaccinated with 5 mg doses of VCL-CB01 at 0, 3, 7, and 28 days (C).