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. 2010 Aug 27;11(9):3309–3051. doi: 10.3390/ijms11093039

Figure 2.

Figure 2

Mutation of p53 eliminates Pgp −mediated repression of pokemon. MDA-MB- 231 cells (p53 mutated) were used to investigate whether mutation of p53 affects the regulation of pokemon by Pgp. (a) Luciferase analysis of pokemon promotor activity in MDA-MB-231 cells transfected with pcDNA3.1 and Pgp expression vector. (b and c) Modulation of pokemon mRNA expression by Pgp in the presence of mutated p53. MDAMB- 231 cells were transfected with Pgp expression vectors. Pgp and pokemon mRNA levels were determined by RT-PCR (b) and real-time PCR analysis (c). (d) Immunoblot of pokemon, p53 and Pgp in MDA-MB-231 cells after transfection with pcDNA 3.1 or Pgp.