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. 2010 Aug 27;11(9):3309–3051. doi: 10.3390/ijms11093039

Table 2.

RT-PCR and ChIP PCR conditions.

Pokemon p53 Pgp GAPDH ChIP PCR

Temp. (°C) Time (min:sec) Cycle Temp. (°C) Time (min:sec) Cycle Temp. (°C) Time (min:sec) Cycle Temp. (°C) Time (min:sec) Cycle Temp. (°C) Time (min:sec) Cycle
98 02:40 1 98 02:40 1 98 02:40 1 98 02:40 1 98 02:20 1
98 00:15 }26 98 00:15 }26 98 00:15 }26 98 00:15 }26 98 00:18 }27
60 01:00 60 01:00 60 01:00 60 01:00 60 00:30
72 01:30 72 01:30 72 01:30 72 01:30 72 00:30
72 06:00 1 72 06:00 1 72 06:00 1 72 06:00 1 72 10:00 1