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Illustration of experimental setup of the falling vesicle method used to estimate the encapsulation fraction of vesicles. (A) Focus of the microscope objective is offset a given distance prior to vesicle formation. (B) The fall time of the vesicle is used to estimate the density of vesicles. Glucose and sucrose solutions of known density and viscosities are used, and the fraction of each solution encapsulated in the vesicle is determined.
