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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Am J Transplant. 2010 Sep;10(9):1970–1980. doi: 10.1111/j.1600-6143.2010.03223.x

Figure 3. Proliferation and survival of activated T cells in adoptive hosts.

Figure 3

Activated CD4+ or CD8+ CD44hi T cells and B220+ B cells were sorted from SP and LN cells of immunized CD45.1 mice (BALB/c splenocytes, 3 × 107) at 8-days. Sorted CD45.1 CD4+ and CD8+ CD44hi T cells (1 × 106) were CFSE labeled and transferred with or without B220+ B cells (1.5 × 107) into µMT and wt adoptive hosts. At 2-weeks after transfer, CFSE dilution was analyzed on harvested SP and LN cells after gating on CD4+ or CD8+ CD45.1+ T cells. Representative histogram overlays of CFSE dilution in CD4+ or CD8+ CD45.1+ T cells from harvested spleens of µMT and wt adoptive hosts are shown (A). B. B cells help proliferation of activated CD4 T cells in adoptive hosts. Proliferation index is calculated using Flowjo software after gating on CD4+ or CD8+ CD45.1+ T cells (Mean ± SD; *, p < 0.05; n = 3 – 4 mice/grp). C. Apoptosis of activated T cells in adoptive hosts. Harvested SP and LN cells from µMT and wt adoptive hosts were examined for Annexin V+ staining after gating on CD4+ or CD8+ CD45.1+ T cells (Mean ± SD; n = 3 – 4 mice/grp). D. Bcl-2 expression in CD4+ and CD8+ CD45.1+ T cells in adoptive hosts. Representative histogram overlays of Bcl-2 expression are shown after intracellular staining and gating on harvested CD4+ or CD8+ CD45.1+ T cells from adoptive hosts. E. Bcl-2 expression is upregulated in developing CD8 memory T cells in adoptive hosts harboring co-transferred B cells. Median fluorescence intensity of Bcl-2 expression on CD8+ CD45.1+ T cells harvested from adoptive hosts is shown in comparison to isotype control (Mean ± SD; *, p < 0.05, n = 3 – 4 mice/grp).