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. 2010 Oct 15;24(20):2233–2238. doi: 10.1101/gad.1988010

Figure 1.

Figure 1.

Region-specific NSCs from a PN1 mouse brain. Horizontal low-magnification H&E staining from a PN1 mouse brain. The large arrow points to the brainstem and the small arrow points to the cortex. NF1−/− NSCs isolated from a PN1 brainstem and cultured in serum-free conditions with FGF-2/EGF had increased pAKT (S473), increased p-p27 (S10 and T198), increased Olig-2-expressing cells, and increased proliferation rate relative to NF1+/+ NSCs isolated from a PN1 brainstem. In contrast, NF1−/− NSCs isolated from a PN1 cortex had similar levels of pAKT (S473), increased total p27 protein levels, similar number of Olig-2-expressing cells, and similar proliferation rate relative to NF1+/+ NSCs isolated from a PN1 cortex. (Image courtesy of Massimo Squatrito.)