Figure 3.
ChIP analysis of Pol II and dCDK12. (A) ChIP analysis of dCDK12 on Hsp70 using NHS and 10-min heat-shocked (10'HS) samples. (B) ChIP analysis of RNAPII (anti-Rpb3) on Hsp70, as in A. (C) Ratios of dCDK12/RNAPII values on 10-min heat-shocked Hsp70. (D) ChIP analysis of dCDK12 on four constitutively active genes under non-heat-shock conditions. (E) ChIP analysis of RNAPII (anti-Rpb3) on four constitutively active genes under non-heat-shock conditions. (F) Ratios of dCDK12/RNAPII values on four constitutively active genes under non-heat-shock conditions. Measurements in A–F are averages of three biological replicates with standard errors.