Figure 6.

Complementation of bur1 ts allele by chimeric (yeast/human) kinases. (A) The bur1-8 (ts) yeast strain was transformed by empty vector or by vector carrying the 1/9 chimeric kinase construct (cf. Supplemental Fig. S3), and independent transformants were tested for growth at 30°C and 37°C (fivefold serial dilutions spotted in rows). (WT) BUR1⁺ control strain. (B) The bur1-8 (ts) strain was transformed by empty vector or by vector carrying the 1/13 chimeric kinase construct, and independent transformants were tested for growth at 30°C and 37°C (fivefold serial dilutions spotted in rows). (WT) BUR1⁺ control strain.