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. 2010 Oct;26(5):389–397. doi: 10.1089/jop.2010.0072

Table 2.

Inhibition of Thrombin-Mediated Elevation in [Ca2+]i

Treatment [Ca2+]i nM, mean ± SEM Number of cells (n)
Baseline 64 ± 5 79
Thrombin 10 nM 2,141 ± 277a 79
Baseline 124 ± 12 17
Hirudin + thrombin (10 nM each) 165 ± 16 17
Baseline 64 ± 5 16
Hirudin + thrombin (25 nM each) 109 ± 5 16
Baseline/U73122 10 μM 53 ± 5 16
U73122 + thrombin 10 nM 53 ± 4 16
Baseline 94 ± 66 29
Thrombin (10 nM) 1,120 ± 652a 29
Baseline/Y27632 10 μM 50 ± 39 38
Y27632 + thrombin 1,027 ± 550a 38

Hirudin (antithrombin) was preincubated with thrombin for 1 h at room temperature at equimolar or 3-fold higher concentration. In U73122 studies, cells were preincubated with 10 μM U73122 for 20 min before thrombin addition.

a

Statistical significance between baseline, peak, and 1 min postpeak (not shown) mean values, performed by one-way repeated measures ANOVA and SNK method for multiple pair-wise comparison (P < 0.001).