Table 2.

Inhibition of Thrombin-Mediated Elevation in [Ca²⁺]_i

Treatment	[Ca2+]i nM, mean ± SEM	Number of cells (n)
Baseline	64 ± 5	79
Thrombin 10 nM	2,141 ± 277a	79
Baseline	124 ± 12	17
Hirudin + thrombin (10 nM each)	165 ± 16	17
Baseline	64 ± 5	16
Hirudin + thrombin (25 nM each)	109 ± 5	16
Baseline/U73122 10 μM	53 ± 5	16
U73122 + thrombin 10 nM	53 ± 4	16
Baseline	94 ± 66	29
Thrombin (10 nM)	1,120 ± 652a	29
Baseline/Y27632 10 μM	50 ± 39	38
Y27632 + thrombin	1,027 ± 550a	38

Hirudin (antithrombin) was preincubated with thrombin for 1 h at room temperature at equimolar or 3-fold higher concentration. In U73122 studies, cells were preincubated with 10 μM U73122 for 20 min before thrombin addition.

^aStatistical significance between baseline, peak, and 1 min postpeak (not shown) mean values, performed by one-way repeated measures ANOVA and SNK method for multiple pair-wise comparison (P < 0.001).