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. Author manuscript; available in PMC: 2011 Sep 24.
Published in final edited form as: Vaccine. 2010 Aug 13;28(41):6675–6683. doi: 10.1016/j.vaccine.2010.08.012

FIGURE 2.

FIGURE 2

DC-directed IDLVs mediated efficient transduction and targeted DCs in vitro. (A). 293T.hDC-SIGN cells were spin-transduced with 30ng p24 of fresh viral supernatant of FUGW/SVGmu(IN−) or FUGW/SVGmu(IN+), and the GFP expression was measured over time by flow cytometry. Transduction in 293T cells served as a negative control. (B) and (C). Bone marrow cells harvested from naïve C57BL/6 mice were cultured in the presence of GM-CSF for 6 days to differentiate bone marrow derived dendritic cells (BMDCs). BMDCs were exposed to 40ng p24 of fresh viral supernatant of FUGW/SVGmu(IN−) or FUGW/SVGmu(IN+), and the variation of GFP expression over the time was recorded in (B). The co-expression of GFP and CD11c at 7 days post-transduction is shown in (C). Transduction of BMDCs with the VSVG-pseudotyped lentiviral vector (FUGW/VSVG(IN+)) was included as a non-DC-targeting control. (D). Total bone marrow cells were harvested and cultured overnight in presence of GM-CSF. On the following day, bone marrow cells were spin-transduced with 600ng p24 of concentrated FUGW/SVGmu(IN−) or FUGW/SVGmu(IN+). Two days later, the expression of GFP and CD11c was analyzed by flow cytometry.