FIGURE 5.

Effective antigen-specific anti-tumor immunity was generated by a single injection of the DC-directed IDLV. Naïve C57BL/6 mice were immunized with FKOVA/SVGmu(IN−), FKOVA/SVGmu(IN+), or the empty vector FUW/SVGmu(IN+) (denoted as Mock). The amount of viral particles injected into each mouse was measured as approximately 800 ng p24. Mice without immunization (represented as No IM) were included as a negative control. Two weeks later, each mouse was subcutaneously transplanted with 5×10⁶ tumor cells of either EG.7 cells expressing OVA or EL4 cells lacking OVA expression. Tumor growth was monitored by calculating the product of the largest perpendicular length and width measured with a fine caliper. Each group was composed of four mice. (A). The growth curve of EG.7 tumors in the treated or untreated mice. (B). The growth curve of EL4 tumors in the treated or untreated mice. (C). Representative data for the frequency of OVA-specific CD8 T cells in the EG.7 tumor-challenged mice. (D). Representative data for the frequency of OVA-specific CD8 T cells in the EL4 tumor-challenged mice. The error bars shown in this figure represent deviation between mice within a group.