Table 1. Primers used for amplifying pandemic H1N1/2009 viral segments.
| Segment | Primera | Sequence |
|---|---|---|
| PB2 | PB2-1877F | 5′-AACTTCTCCCCTTTGCTGCT-3′ |
| PB2-2062R | 5′-GATCTTCAGTCAATGCACCTG-3′ | |
| PB1 | PB1-825F | 5′-ACAGTCTGGGCTCCCAGTA-3′ |
| PB1-1138R | 5′-TTTCTGCTGGTATTTGTGTTCGAA-3′ | |
| PA | PA-821F | 5′-GCCCCCTCAGATTGCCTG-3′ |
| PA-1239R | 5′-GCTTGCTAGAGATCTGGGC-3′ | |
| HA | HA-398F | 5′-GAGCTCAGTGTCATCATTTGAA-3′ |
| HA-570R | 5′-TGCTGAGCTTTGGGTATGAA-3′ | |
| NP | NP-593F | 5′-TGAAAGGAGTTGGAACAATAGCAA-3′ |
| NP-942R | 5′-GACCAGTGAGTACCCTTCCC-3′ | |
| NA | NA-163F | 5′-CATGCAATCAAAGCGTCATT-3′ |
| NA-268R | 5′-ACGGAAACCACTGACTGTCC-3′ | |
| M | M-504F | 5′-GGTCTCACAGACAGATGGCT-3′ |
| M-818R | 5′-GATCCCAATGATATTTGCTGCAATG-3′ | |
| NS | NS-252F | 5′-ACACTTAGAATGACAATTGCATCTGT-3′ |
| NS-691R | 5′-ACTTTTCATTTCTGCTCTGGAGGT-3′ |
a
Number indicates the nucleotide position of the first base in the target sequence (cRNA sense).