Table 2.
Only one SNP, rs9296068 near the HLA-DOA gene, met criteria for selection and hypothesis testing in our two-tier approach. This SNP demonstrated a large difference (p=0.041) in MAF when parents of Rejectors were compared with parents of Non-Rejectors, as well as significant differences in parent-to-child transmission between Rejectors and Non-Rejectors, on the GC test (p=0.0183). Transmission data show more parents (n=19) transmitting the minor allele of rs9296068 to their Rejector offspring than parents who did not (n=12). The reverse was true among Non-Rejectors, whose parents were less likely to transmit this risk allele (7 transmissions vs 17 non-transmissions).
| HLA-DOA/ SNP=rs9296068 (physical position=33,096,673) | |
|---|---|
| Rejector vs. Non-Rejector MAF p value | 0.041 |
| Rejector vs. Non-Rejector GC p value | 0.018 |
| GC transmission parameter (τ2)† | 1.9 |
| Allele | 2 |
| Rejector parents | |
| MAF ‡ | 46.3% |
| Number transmitted | 19 |
| Number non-transmitted | 12 |
| Non-Rejector parents | |
| MAF‡ | 33.8% |
| Number transmitted | 7 |
| Number non-transmitted | 17 |
MAF=minor allele frequency
For the GC statistic, under the null hypothesis of Mendelian segregation, τk=1 for all k alleles (11)