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. Author manuscript; available in PMC: 2010 Oct 18.
Published in final edited form as: Nat Genet. 2010 Feb 7;42(3):216–223. doi: 10.1038/ng.527

Figure 6. DOK2 suppresses lung cancer cell proliferation in vitro and in vivo.

Figure 6

(a) Growth curve analysis of H1299 cells with and without retroviral-mediated overexpression of DOK2. Relative cell number was determined after cell fixation and crystal violet staining using the optical density at 595 nm (OD 595). Triplicate wells were performed and the data shown is mean+SD of a representative experiment. The experiment was repeated five times. A Western blot, inset, confirms expression of DOK2. (b) Analysis of Erk and Akt activation in the same cells used for the experiment in panel (a). Cells were serum starved in medium containing 0.1% FCS for 12 hours, stimulated with 20% FCS for the times indicated (minutes), then lysed and used for Western blot analysis with antibodies against phosphorylated Erk1/2 (pErk) or phosphorylated Akt (pAkt). (c) Tumor volume measurements of tumors formed from subcutaneous injection of H1299 cells with or without DOK2 expression into the flanks of nude mice. The experiment was performed in triplicate and the data shown is mean+SD. A Western blot, inset, confirms expression of DOK2. (d) Picture of tumors formed by H1299 cells with or without expression of DOK2 taken at week 5 after cell injection. An arrowhead indicates the tumor mass in each panel.