Fig. 4.

Protein levels of VEGF in the vitreous humor. VEGF protein levels in vitreous humor from sham-operated and ischemia-reperfusion eyes. Extracts of vitreous humor were incubated on an angiogenesis antibody array membrane (see “Materials and Methods”). The upper panel shows a representative example of the array membrane after incubation with vitreous humor. The bottom panel shows the mean values ± SEM obtained from the quantification of the spot intensity (n = 6). Statistical analysis was performed using paired Student’s ratio t test. Note that VEGF protein levels in the vitreous humor are higher following ischemia reperfusion